Part:BBa_K2331004:Design

Periplasmic-Localizing Endoglucanase with HIS-Tag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 87
  Illegal XhoI site found at 1153
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 54
  Illegal AgeI site found at 308
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

A codon before the his-tag had to be modified to get rid of an illegal NotI site.

Source

This part was synthesized de novo from Ruminiclostridium thermocellum.